Tricaine-S

Generic name: tricaine powder, for solution
Dosage form: FOR ANIMAL USE ONLY

Tricaine-S
(tricaine methanesulfonate)

KEEP TIGHTLY CLOSED USE ONLY FRESH SOLUTION

Before using this drug, read package insert for complete
product information.

Store at room temperature (Approximately 25°C (77°F))

KEEP OUT OF THE REACH OF CHILDREN

Tricaine-S is intended for the temporary immobilization of fish, amphibians, and other aquatic, cold-blooded animals (poikilotherms) as an aid in handling during manual spawning (fish stripping), weighing, measuring, marking, surgical operations, transport, photography, and research.

Warnings

WARNINGS

Do not use within 21 days of harvesting fish for food. Use in fish intended for food should be restricted to Ictaluridae, Salmonidae, Esocidae, and Percidae, and water temperature exceeding 10ºC (50ºF). In other fish and in cold-blooded animals, Tricaine-S should be limited to hatchery or laboratory use. Avoid inhaling or getting into eyes.

Chemistry

Tricaine-S is the methanesulfonate of meta-amino benzoic acid ethylester, or simply ethyl m-amino benzoate. It is thus an isomer of benzocaine having the formula C9H11O2N + CH3SO3H

Tricaine-S is a fine white crystalline powder. Its molecular weight is 261.3. Soluble to 11%, it forms clear, colorless acid solutions in water.

Toxicology

Comparative toxicologic studies carried out on fish and frogs gave the following results:
FISH TOXCITY STUDIES - The toxicity of Tricaine-S was measured by standard methods in laboratory bioassays with rainbow trout, brown trout, brook trout, lake trout, northern pike, channel catfish, bluegill, largemoth bass and walleye. The 24, 48 and 96 hour LC50 (lethal concentration for 50 percent of the animals) values for trout ranged from 52 to 31 mg/liter; for northern pike, from 56 to 48 mg/liter; for catfish, from 66 to 50 mg/liter; for bluegill and largemouth bass, from 61 to 39 mg/liter; and for walleye, the values were 49 to 46 mg/liter.
Safety Index:The safety indices for Tricaine-S refer to the margin between concentrations which cause anesthesia and mortality. They are expressed by the quotient of the lethal concentration for 50 percent of the fish (LC50) and the effective concentration for 50 percent of the fish (EC50)

Safety Indices for Rainbow Trout and Channel Catfish at 12°C (54°F)

FROG TOXICITY STUDIES3 - Frogs were put into various concentrations of Tricaine-S for 30 minutes and the